Device

Part:BBa_K4062026:Design

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-16)


lactose regulated bla, lacI ORF mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2027


Design Notes

The third and fourth codons of the lacI ORF have been replaced by stop codons. lacI will not be expressed and thus its regulatory effects not observed.


Source

Sequences from the genomic library of Escherichia coli K12 from biocyc.org.

References